The media were replaced daily, until day 6 when culture media were changed to neuronal media containing Neurobasal-A (Gibco/Fisher, Cat# 10-888-022) supplemented with 2% Gibco B-27 serum-free supplement without vitamin A (Fisher Scientific, Cat# 12-587-010), 1% GlutaMAX Supplement (Gibco/Fisher, Cat# 35-050-061), and 1% penicillin/streptomycin (Gibco/Fisher Scientific, Cat# 15-140-122)
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Papers and reviews have examined it in relation to tendon healing , muscle injury , ligament-related questions , gastrointestinal tissue , and broader wound-healing frameworks
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